Prototyping of lactate biosensor for non-invasive biomarker monitoring
Leino, Liisa (2023)
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi-fe20231122148486
https://urn.fi/URN:NBN:fi-fe20231122148486
Tiivistelmä
Biosensors are widely used in diagnostics and health monitoring in different applications. They are simple, and cost-effective tools to monitor health and diseases and, therefore, they have attracted significant interest especially during the recent years. Lactate biosensors have got into focus since lactate is an important parameter of health and fitness.
The objective of this thesis was to prepare prototypes of lactate biosensors for non-invasive biomarker monitoring. Lactate oxidase was used as a biorecognition element, which produces H2O2 during the conversion of lactate and oxygen that can be measured amperometrically. Different immobilization methods were used to investigate the possibilities of increasing the long-term stability and sensitivity of biosensors. For that, the enzyme was entrapped in a chitosan and in a Nafion matrix, it was immobilized with PEGDE and cross-linked with GA.
Three of the different methods gave promising results and with further optimizations, it is possible to increase the stability and sensitivity even further.
The objective of this thesis was to prepare prototypes of lactate biosensors for non-invasive biomarker monitoring. Lactate oxidase was used as a biorecognition element, which produces H2O2 during the conversion of lactate and oxygen that can be measured amperometrically. Different immobilization methods were used to investigate the possibilities of increasing the long-term stability and sensitivity of biosensors. For that, the enzyme was entrapped in a chitosan and in a Nafion matrix, it was immobilized with PEGDE and cross-linked with GA.
Three of the different methods gave promising results and with further optimizations, it is possible to increase the stability and sensitivity even further.