Mesoporous silica nanoparticles as protein carriers for antibiofilm applications
Peura, Kia (2022)
Peura, Kia
2022
Julkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty.
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi-fe2022042831411
https://urn.fi/URN:NBN:fi-fe2022042831411
Tiivistelmä
Multidrug resistant (MDR) bacteria are currently one of the biggest public health threats. About 60 to 70% of bacterial infections are associated with formation of biofilms that are a cause of a wide variety of chronic infections. Biofilms are bacterial communities embedded within an extracellular polymeric substance matrix (EPS) which consists of lipids, polysaccharides, proteins, and nucleic acids. Biofilms protect bacteria effectively from external threats, such as antibacterial agents. Current antimicrobial research aims to affect the structure of the biofilm and, in this way, enhance the effects of antibacterial agents.
In this thesis, large pore (10 nm) mesoporous silica nanoparticles (MSNs) were examined as potential antibacterial protein carriers for antibiofilm applications. A loading protocol for proteins with similar sizes to the used model protein, bovine serum albumin (BSA), resulted in an average loading capacity of 30%. Biofilm formation and the changes in biofilm growth was studied to validate that MSNs do not have intrinsic antimicrobial effects. Short-term in vitro protein release studies conducted in pHs corresponding to the pH gradient within biofilms unveiled premature release of protein cargo.
This project demonstrated that MSNs can be a potential drug delivery system (DDS) for therapeutic proteins and could be exploited for antibiofilm applications. The use of nanosized drug delivery systems (DDSs) could improve penetration and accumulation of antibiotics in biofilms and provide enhanced efficacy and reduced side-effects of antimicrobial therapeutics.
In this thesis, large pore (10 nm) mesoporous silica nanoparticles (MSNs) were examined as potential antibacterial protein carriers for antibiofilm applications. A loading protocol for proteins with similar sizes to the used model protein, bovine serum albumin (BSA), resulted in an average loading capacity of 30%. Biofilm formation and the changes in biofilm growth was studied to validate that MSNs do not have intrinsic antimicrobial effects. Short-term in vitro protein release studies conducted in pHs corresponding to the pH gradient within biofilms unveiled premature release of protein cargo.
This project demonstrated that MSNs can be a potential drug delivery system (DDS) for therapeutic proteins and could be exploited for antibiofilm applications. The use of nanosized drug delivery systems (DDSs) could improve penetration and accumulation of antibiotics in biofilms and provide enhanced efficacy and reduced side-effects of antimicrobial therapeutics.
Kokoelmat
- 317 Farmasia [19]